Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays
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چکیده
منابع مشابه
Quantitative analyses of electrostatic interactions between NADPH-cytochrome P450 reductase and cytochrome P450 enzymes.
A decline in the ionic interactions in the medium with increasing ionic strength (decrease in the ionic activity coefficients) was accompanied by an increase in the fast phase rate constants of CYP2B4 and CYP1A2 reduction. The stimulations were observed both in reconstituted P450 systems and in microsomes. An increase in the ionic strength from 10 to 100 mM sodium phosphate resulted in a 7-fold...
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Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}-5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerob...
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NADPH oxidation and cytochrome c reduction with and without lucigenin as well as NAD(P)H/lucigenin-dependent chemiluminescence of rat liver microsomes were studied. An increased rate of NADPH oxidation and cytochrome c reduction in the presence of lucigenin was related to one-electron lucigenin reduction by microsomal NADPH reductases. The apparent Michaelis constant values for lucigenin (Km ap...
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Proton channels allow cells to extrude excess acid with a high efficiency and therefore are one of the important homeostatic mechanisms that maintain the intracellular pH in a range compatible with life. Proton currents are highly expressed in phagocytes where they are thought to contribute to charge and pH compensation during NADPH oxidase activation. By analogy with the cytochrome C oxidase o...
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and Duox in the respiratory epithelium. The location of these ROS-generating enzymes in the barrier tissues has prompted the expectation of their participation in host defense against invading pathogens in these tissues. Indeed, recent studies have begun to support this expectation, demonstrating roles in bacterial killing, cytokine production, apoptosis, gene expression and signaling, includin...
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ژورنال
عنوان ژورنال: Free Radical Biology and Medicine
سال: 2017
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2016.11.019